个人简介：

柴迎梅，电话：13173307650，邮箱: 和虾的免疫工作，分子克隆和表达免疫相关蛋白基因并研究其功能，从松江鲈鱼中克隆了fibrinogen相关蛋白基因，白细胞趋化因子等基因。主持纵向课题2项, 其中国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金1项，山东省博士后创新专项资金1项。参与纵向项目3项，其中国家自然基金2项，立博自然基金1项。主持开发类项目4项。发表学术论文20余篇，其中SCI收录15篇。作为第一作者发表SCI论文7篇,其中JCR一区论文5篇，并且该期刊为农林科学大类TOP期刊；

· 2007-2009年,在山东老员工命学院动物实验研究室,做博士后工作，主要研究对虾肝胰腺受白斑病毒感染后的蛋白质组学工作，并克隆对虾的fibrinogen相关蛋白基因。

· 2003-2006在国家海洋局第一海洋研究所海洋活性物质重点实验室攻读博士学位，主要研究大菱鲆细胞分泌惨与免疫功能，并克隆了VAMP-2蛋白基因。

· 2014.3-2014.9在美国耶鲁大学医学院癌症研究中心做各种药物对knock-down ERbB2和3基因后癌细胞影响研究

发表论文：

[1]Yingmei Chai, Bailin Cong, Shanshan Yu, Yingying Liu,Xin Man, Lujie Wand,Qian Zhu. Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin scalping. Fish Shellfish Immunol.2018,74:620-626. doi: 10.1016/j.fsi.2017.12.069.（SCI收录,JCR一区，农林科学大类TOP期刊）

[2] Yingmei Chai,Shanshan Yu, Yingying Liu, Qian Zhu. A fibrinogen-related protein (TfFREP2) gene involving in the immune response of Trachidermus fasciatus against Vibrio anguillarum. Fish Shellfish Immunol. 2013,35(6):1988-92. doi: 10.1016/j.fsi.2013.09.003.（SCI收录,JCR一区，农林科学大类TOP期刊）

[3]Yingmei Chai,Shanshan Yu,Qian Zhu. The molecular cloning and characteristics of a fibrinogen-related protein(TfFREP1) gene from roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2012,33(3):614-8.（SCI收录,JCR一区，农林科学大类TOP期刊）

[4]Yingmei Chai,Qian Zhu,Shanshan Yu, Xiaofan Zhao, Jingxing Wang. A novel protein with a fibrinogen-like domain involved in the innate immune response of Marsupenaeus japonicus. Fish Shellfish Immunol. 2012,32(2):307-15.（SCI收录,JCR一区，农林科学大类TOP期刊）

[5]Yingmei Chai, Xiaohang Huang, Bailin Cong,Chenlin Liu,Shenhao Liu. Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus) Acta Oceanologica Sinica. 2010, 29：116-119. (SCI收录)

[6] Yingmei Chai, Shanshan Yu, Xiaofan Zhao, Qian Zhu, Jinxing Wang. Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to white spot syndrome virus. Fish Shellfish Immunology.2010,29: 480-6.（SCI收录,JCR一区，农林科学大类TOP期刊）

[7]Yingmei Chai, Xiaohang Huang, Bailin Cong, Shenghao Liu, Kui Chen, Guangyou Li, Gaisano Herbert Y. Involvement of VAMP-2 in exocytosis of IL-1b in turbot (Scophthalmus maximus L) leukocytes after Vibrio anguillarum infection. Biochemical and Biophysical Research Communications. 2006, 342: (SCI收录)

[8] Bailin Cong,Yingmei Chai, Bo Wang, Shenghao Liu, Nengfei Wang, Quanqi Zhang, Xiaohang Huang. Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas). Fish&Shellfish Immunology, 2017, 60:426-435.（SCI收录,JCR一区，农林科学大类TOP期刊）

[9] Yingying Liu, Xiaodi Han, Xuezhao Chen, Shanshan Yu,Yingmei Chai, Ttongjie Zhai, Qian Zhu. Molecular characterization and functional analysis of the hepcidin gene from roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2017，68:349-358. doi: 10.1016/j.fsi.2017.07.044.（SCI收录,JCR一区，农林科学大类TOP期刊）

[10] Shanshan Yu, Hui Yang,Yingmei Chai, Yingying Liu, Qiuxia Zhang, Xinbiao Ding, Qian Zhu. Molecular cloning and characterization of a C-type lectin in roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2013，34(2):582-92. doi: 10.1016/j.fsi.2012.11.033.（SCI收录,JCR一区，农林科学大类TOP期刊）

[11] Hui Yang, Shanshan Yu,Yingmei Chai, Yingying Liu, Qiuxia Zhang, Xinbiao Ding, Qian Zhu. A galectin from roughskin sculpin, Trachidermus fasciatus: molecular cloning and characterization. Fish Shellfish Immunol. 2013，35(3):815-24. doi: 10.1016/j.fsi.2013.06.015.（SCI收录,JCR一区，农林科学大类TOP期刊）

[12] Yingying Liu, Shanshan Yu,Yingmei Chai, Qiuxia Zhang, Hui Yang, Qian Zhu. Lipopolysaccharide-induced gene expression of IRAK-4 and IL-1β in roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2012,33(4):690-8.（SCI收录,JCR一区，农林科学大类TOP期刊）

[13] Yingying Liu, Shanshan Yu,Yingmei Chai, Qian Zhu. Transferrin gene expression in response to LPS challenge and heavy metal exposure in roughskin sculpin (Trachidermus fasciatus).Fish Shellfish Immunol. 2012,32(1):223-9.（SCI收录,JCR一区，农林科学大类TOP期刊）

[14] Kui Chen, Xiaohang Huang,Yingmei Chai, Gaisano Herbert Y. Characterization of SNAP-25 gene from marine teleostean,Lateolabrax japonicus. Chinese Journal of Oceanology and Limnology. 2007,25:378-5. (SCI收录)

[15] Shenghao Liu, Chenlin Liu, Xiaohang Huang, Yingmei Chai, Bailin Cong. Optimization of parameters for isolation of protoplasts from the Antarctic sea ice alga Chlamydomonas sp. ICE-L. Journal Applied Phycology. 2006, 18: 783-786. (SCI收录)

[16]周瑜,柴迎梅,杜宗军,刘骏.海洋石油降解菌的分离、鉴定和与微藻共培养海洋环境科学.2011;30（2）：230-3.（通讯作者）

[17]柴迎梅,黄晓航,刘晨临,丛柏林,刘胜浩.淋巴细胞中钙信号的研究进展.免疫学杂志.2005;21(3):50-3

[18] 柴迎梅,刘胜浩,丛柏林,黄晓航. VAMP-2在鲈鱼组织中的表达分布.海洋科学进展, 2005, 23(B12):6-10.

[19]柴迎梅;曹殿军;闫丽辉;刘培欣;黄永芬;汪清胤.新城疫病毒F48E9株NP基因的克隆与序列分析.中国预防兽医学报.2002;24(2):110-2.

[20]柴迎梅,曹殿军.核衣壳蛋白及“六碱基规则”在新城疫病毒复制中的作用.中国预防兽医学报.2002;24(3):235-6.

[21]黄晓航,柴迎梅,刘晨临,丛柏林,刘胜浩.膜片钳技术在海洋鱼类研究中的应用与展望.中国海洋大学学报(自然科学版).2006;36(2):221-4.

[22]刘胜浩,刘晨临,黄晓航,柴迎梅,丛柏林.植物细胞的非选择性阳离子通道植物生理学通讯,2006;42(3):523-8.

[23]丛柏林, 黄晓航,柴迎梅,刘胜浩,刘晨临.孔鳐胰脏单层细胞的制备和外源基因的表达.海洋科学进展, 2005, 23(B12):19-23.

Curriculum vitae：

Yingmei Chai,Phone:13173307650,Email:chaiyingmei@sdu.edu.cn

PROFESSIONAL EXPERIENCES

July 2001-Present, Shandong University, Weihai, Shandong, China, Associated professor

Teach biochemical theory and experiments. Do research on a novel protein with a fibrinogen-like domain inMarsupenaeus japonicas.Perform gene cloning and protein expression profile by quantitative real-time polymerase chain reaction, express the recombinant protein and make antiserum to be used in the protein functional analysis. Clone and express two fibrinogen-related proteinsand LECT2 genein roughskinsculpin (Trachidermusfasciatus).

Hostedtwoprojects, includingone open fund of key laboratory of Marine biological active substance and modern analysis technology of state oceanic administration, andone special fund of postdoctoral innovation of shandong province. Participated inthreeprojects, among whichtwo were national natural fund andone was natural fund of shandong university. Hostingfourdevelopment projects.Published over 20 academic papers, among which 15 have been included in SCI. As the first author, I published 7 SCI papers, including 5 JCR papers in the first district, and this journal is the TOP journal of agricultural and forestry science.

Sep 2007-May 2011, Shandong University, Jinan, Shandong, China, postdoctoral research person

Analyze comparative proteomic of the hepatopancreas inFenneropenaeuschinensis responseto white spot syndromevirus by 2-D Gel.

Sep 2003-Jun 2006, Ocean University of China, Qingdao, Shandong, China, PH.D.

Clone VAMP-2 protein gene of turbot and study the function of VAMP-2 in the secretion of interlukin-1β.

Mar 2014-Sep 2014. Yale school of medicine, Yale University, New Haven, USA.

Study the role of ERBB2 and ERBB3 in drug resistance in EGFR mutant lung cancer.

PUBLICATIONS

[1]Yingmei Chai, Bailin Cong, Shanshan Yu, Yingying Liu,Xin Man, Lujie Wand,Qian Zhu. Effect of a LECT2 on the immune response of peritoneal lecukocytes against Vibrio anguillarum in roughskin scalping. Fish Shellfish Immunol.2018,74:620-626. doi: 10.1016/j.fsi.2017.12.069.（SCI）

[2] Yingmei Chai, Shanshan Yu, Yingying Liu, Qian Zhu. A fibrinogen-related protein (TfFREP2) gene involving in the immune response of Trachidermus fasciatus against Vibrio anguillarum. Fish Shellfish Immunol. 2013,35(6):1988-92. doi: 10.1016/j.fsi.2013.09.003.（SCI）

[3]Yingmei Chai, Shanshan Yu, Qian Zhu. The molecular cloning and characteristics of a fibrinogen-related protein(TfFREP1) gene from roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2012,33(3):614-8.（SCI）

[4]Yingmei Chai,Qian Zhu,Shanshan Yu, Xiaofan Zhao, Jingxing Wang. A novel protein with a fibrinogen-like domain involved in the innate immune response of Marsupenaeus japonicus. Fish Shellfish Immunol. 2012,32(2):307-15.（SCI）

[5]Yingmei Chai, Xiaohang Huang, Bailin Cong,Chenlin Liu,Shenhao Liu. Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus) Acta Oceanologica Sinica. 2010, 29：116-119. (SCI收录)

[6] Yingmei Chai, Shanshan Yu, Xiaofan Zhao, Qian Zhu, Jinxing Wang. Comparative proteomic profiles of the hepatopancreas in Fenneropenaeus chinensis response to white spot syndrome virus. Fish Shellfish Immunology.2010,29: 480-6.（SCI）

[7]Yingmei Chai, Xiaohang Huang, Bailin Cong, Shenghao Liu, Kui Chen, Guangyou Li, Gaisano Herbert Y. Involvement of VAMP-2 in exocytosis of IL-1b in turbot (Scophthalmus maximus L) leukocytes after Vibrio anguillarum infection. Biochemical and Biophysical Research Communications. 2006, 342: (SCI)

[8] Bailin Cong,Yingmei Chai, Bo Wang, Shenghao Liu, Nengfei Wang, Quanqi Zhang, Xiaohang Huang. Molecular characterization and functional analysis of four teleostean K+ channels in macrophages of sea perch (Lateolabrax japonicas). Fish&Shellfish Immunology, 2017, 60:426-435.（SCI）

[9] Yingying Liu, Xiaodi Han, Xuezhao Chen, Shanshan Yu,Yingmei Chai, Ttongjie Zhai, Qian Zhu. Molecular characterization and functional analysis of the hepcidin gene from roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol.2017，68:349-358.（SCI）

[10] Shanshan Yu, Hui Yang,Yingmei Chai, Yingying Liu, Qiuxia Zhang, Xinbiao Ding, Qian Zhu. Molecular cloning and characterization of a C-type lectin in roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2013，34(2):582-92.（SCI）

[11] Hui Yang, Shanshan Yu,Yingmei Chai, Yingying Liu, Qiuxia Zhang, Xinbiao Ding, Qian Zhu. A galectin from roughskin sculpin, Trachidermus fasciatus: molecular cloning and characterization. Fish Shellfish Immunol. 2013，35(3):815-24.（SCI）

[12] Yingying Liu, Shanshan Yu,Yingmei Chai, Qiuxia Zhang, Hui Yang, Qian Zhu. Lipopolysaccharide-induced gene expression of IRAK-4 and IL-1β in roughskin sculpin (Trachidermus fasciatus). Fish Shellfish Immunol. 2012,33(4):690-8.（SCI）

[13] Yingying Liu, Shanshan Yu,Yingmei Chai, Qian Zhu. Transferrin gene expression in response to LPS challenge and heavy metal exposure in roughskin sculpin (Trachidermus fasciatus).Fish Shellfish Immunol.2012,32(1):223-9.（SCI）

[14] Kui Chen, Xiaohang Huang,Yingmei Chai, Gaisano Herbert Y. Characterization of SNAP-25 gene from marine teleostean,Lateolabrax japonicus. Chinese Journal of Oceanology and Limnology. 2007,25:378-5. (SCI)

[15] Shenghao Liu, Chenlin Liu, Xiaohang Huang, Yingmei Chai, Bailin Cong. Optimization of parameters for isolation of protoplasts from the Antarctic sea ice alga Chlamydomonas sp. ICE-L. Journal Applied Phycology. 2006, 18: 783-786. (SCI)